Mapping in vivo associations of cytoplasmic proteins with integrin beta 1 cytoplasmic domain mutants.
نویسندگان
چکیده
منابع مشابه
Integrin cytoplasmic domain-binding proteins.
Integrins are a large family of cell surface receptors that mediate cell adhesion and influence migration, signal transduction, and gene expression. The cytoplasmic domains of integrins play a pivotal role in these integrin-mediated cellular functions. Through interaction with the cytoskeleton, signaling molecules, and other cellular proteins, integrin cytoplasmic domains transduce signals from...
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The integrin family of cell surface receptors can be divided into three groups on the basis of their homologous beta subunits: beta 1, beta 2, and beta 3. We have raised an antibody against a synthetic peptide corresponding to the COOH-terminal domain of the chicken integrin beta 1 subunit that reacts with beta subunits from a variety of vertebrates, invertebrates, and fungi, demonstrating stro...
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Article Chronology:Received 28 March 2006Revised version received6 June 2006Accepted 13 June 2006Available online 29 June 2006The three Kindlins are a novel family of focal adhesion proteins. The Kindlin-1 (URP1) gene ismutated in Kindler syndrome, the first skin blistering disease affecting actin attachment inbasal keratinocytes. Kindlin-2 (Mig-2), the best stud...
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Chicken integrin beta 1 cDNA and its site-directed mutants were cloned into a mammalian expression vector and introduced into mouse NIH 3T3 cells. Stable transfectants expressing the chicken beta 1 subunit or its site-directed mutants were identified by immunostaining with antibodies specific for the chicken integrin beta 1 subunit. The chicken beta 1 proteins were expressed predominately in th...
متن کاملIdentification of amino acid sequences in the integrin beta 1 cytoplasmic domain implicated in cytoskeletal association
Wild-type and mutant chicken integrin beta 1 subunit (beta 1c) cDNAs were expressed in NIH 3T3 cells and assayed for localization in focal adhesions of cells plated on fibronectin substrates. Focal adhesion localization in stable transfected cells was assayed by indirect immunofluorescent staining with chicken-specific anti-beta 1c antibodies. Mutant beta 1c integrins containing internal deleti...
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ژورنال
عنوان ژورنال: Molecular Biology of the Cell
سال: 1995
ISSN: 1059-1524,1939-4586
DOI: 10.1091/mbc.6.2.151